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Cryopreservation of Haptenized Tumor Cells
专利权人:
David Berd
发明人:
David Berd,Lorne F. Erdile,Margalit B. Mokyr
申请号:
US13888024
公开号:
US20140113371A1
申请日:
2013.05.06
申请国别(地区):
US
年份:
2014
代理人:
摘要:
A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hanks buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.
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