The present invention provides the novel sulfated polysaccharides with anticoagulant active. Specifically, the polysaccharide the present invention provides the repetitive structure of the disaccharide unit comprising being made of hexuronic acid (HexA) residue and alpha-D aminoglucose (GlcN) residue and in GlcN residues with 13% or higher 3-O- sulphations ratio.