A method of manufacturing a composition for enlarging the penis comprises the steps of picking the auto-tissue of a skin from the abdomen or flank region of a patient； (2) obtaining a purified mixture of the dermis and fat by removing the red blood cells of a lower layer from the auto-tissue through centrifugation； (3) separating and extracting stem cells from part of the purified mixture； (4) picking blood from the patient and purifying the blood through centrifugation； (5) extracting a Platelet Rich Plasma (PRP), including rich growth factors, from the purified blood； and (6) fabricating the composition by mixing the mixture obtained at the step (2) and not used to extract the stem cells, the stem cells, and the Platelet Rich Plasma (PRP).
