Cells are cultured on a cell culture support having a substrate surface coated with a temperature-responsive polymer whose upper or lower critical solution temperature in water is 0 SIMILAR 80 DEG C, and subsequently, (1) the temperature of the culture solution is brought to above the upper critical solution temperature or below the lower critical solution temperature, and optionally, (2) the cultured cell sheet is brought into close contact with a polymer membrane and (3) the cell sheet is peeled off together with the polymer membrane. In this way, the myocardial cell sheet can be cultured three-dimensionally to construct a myocardium-like tissue.
