Technologies are generally described for providing medical imaging during radiation therapy. In one embodiment, a radiation therapy system includes an x-ray apparatus and a monitoring apparatus. The x-ray apparatus is configured for delivery of a therapeutic beam of radiation to tissue of a patient. The monitoring apparatus is configured for monitoring production of free radicals within the tissue during the delivery in order to determine the path taken by the x-ray beam of radiation through the tissue. A computer program product for controlling radiation therapy is provided. Methods for calibrating the radiation therapy system and for providing radiation therapy are included. In some embodiments, the monitoring apparatus may be configured for monitoring production of biomarkers within the tissue, the biomarkers including at least one of free radicals, burst vesicles, dissociation of an adduct and an indication of protein damage.
