The present invention provides a stroboscopic optical image mapping system comprising a control module, an optical module, and an image acquiring unit. The control unit forms a delay signal by modulating a first pulse signal having a plurality of pulses with a period, wherein a time interval between two adjacent pulses of the delay signal has a time difference with respect to the period. The optical module provides an incident light projecting on an organic object, which has a dye therein and is stimulated by a second pulse signal for generating a sequential action potential. The incident light excites the dye inside the organic object so that the organic object generates a fluorescent light corresponding to the intensity of the sequential action potential. The image acquiring unit is actuated to acquire the fluorescent light according to the delay signal thereby forming a plurality of fluorescent images.
