Disclosed is a Hepatitis C virus (HCV) vaccine comprising at least two epitopes, each from a different hotspot epitope, wherein a hotspot epitope is defined as an epitope containing peptide selected from the group consisting ofKFPGGGQIVGGVYLLPRRGPRLGVRATRK,GYKVLVLNPSVAAT,AYAAQGYKVLVLNPSVAAT,DLMGYIP(A/L)VGAPL,GEVQVVSTATQSFLATCINGVCWTV,HMWNFISGIQYLAGLSTLPGNPA,VDYPYRLWHYPCT(V/I)N(F/Y)TIFK(V/I)RMYVGGVEHRL,AAWYELTPAETTVRLR,GQGWRLLAPITAYSQQTRGLLGCIV,IGLGKVLVDILAGYGAGVAGALVAFK,FTDNSSPPAVPQTFQV,LEDRDRSELSPLLLSTTEW,YLVAYQATVCARAQAPPPSWD,MSTNPKPQRKTKRNTNR,LINTNGSWHINRTALNCNDSL,TTILGIGTVLDQAET,FDS(S/V)VLCECYDAG(A/C)AWYE,ARLIVFPDLGVRVCEKMALY,AFCSAMYVGDLCGSV,GVLFGLAYFSMVGNW,VVCCSMSYTWTGALITPC,TRVPYFVRAQGLIRA andTTLLFNILGGWVAAQ.
