An endoscope system and a light source machine thereof are provided, wherein the endoscope system includes a light source machine, an endoscope and an image capturing device, the light source machine includes a light guiding device and a plurality of independent light source modules, wherein the light source machine is capable of accurately emitting a wave band light source required by an user through the independent light source modules, and guiding the light emitted by the light source modules to the endoscope through the light guiding device. The endoscope is provided with a light source input end, a detecting end and an image output end, wherein the light source input end is connected with a light exit port, and is configured to receive light emitted from the light exit port, and emit the light from the detecting end, so that when the detecting end detects an object to be detected through the light emitted by the light source machine, an image data of the object to be detected can be accurately obtained and output from the image output end.一種內視鏡系統及其光源機，其中，內視鏡系統包含有光源機、內視鏡以及影像擷取裝置，光源機包括有一導光裝置以及複數個獨立光源模組，其中，光源機藉由獨立光源模組可以精確地發出使用者需求的波段光源，並藉由導光裝置引導光源模組所射出的光線至內視鏡。內視鏡具有一光源輸入端、一檢測端以及一影像輸出端，其中，光源輸入端與出光口連接，並且用以接收自出光口所射出之光線，並自檢測端射出，使檢測端藉由光源機所發出之光線檢測一待測標的時，可以準確取得待測標的的影像資料，並自影像輸出端輸出。
