This invention relates to a conical beam tomography scanning equipment and a positioning method thereof, matched up with a positioning system. The positioning system comprises a photographic device, an image processing unit, a control unit, and a driving device. The positioning method comprises the following steps: using the photographic device to shoot an object along a reference axis to generate a reference image using the image processing unit to perform positioning procedure of characteristic points and center point on the reference image to calculate the offset amount of an imaging axis center point of an X-ray image and the reference axis center point using the control unit to convert the offset amount into a driving command and using the driving device to drive an X-ray source, a carrier for fixing the object, and an imaging axis of an X-ray imaging module to be aligned with the reference axis of the photographic device shooting the object according to the driving command.一種錐型光束斷層掃描設備及其定位方法,配合一定位系統,定位系統包括一攝影裝置、一影像處理單元、一控制單元及一驅動裝置,該定位方法包含下述步驟:攝影裝置沿著一參考軸拍攝一物件以產生一參考影像;影像處理單元對於參考影像進行特徵點及參考軸中心點的定位程序以計算X光影像的成像軸中心點及參考軸中心點之偏移量;控制單元將偏移量換算一驅動指令;驅動裝置依據驅動指令驅使X射線源、固定該物件之載台及X射線成像模組之成像軸對準攝影裝置拍攝該物件之參考軸。S201-S204‧‧‧步驟