A dental floss holder includes a shank (11), an adjusting member (12) pivotally mounted on the shank and a cleaning unit (20) detachably mounted on the adjusting member. Thus, the rotation portion (111) of the shank is rotated relative to the mounting portion (122) of the adjusting member to adjust the inclined angle between the shank and the adjusting member so that the inclined angle between the shank and the cleaning unit is adjusted according to a users requirement so as to facilitate the user clearing the teeth in different inclined angles.
