A dental floss member and a dental floss holder are disclosed. A dental floss member comprises: a dental floss which has a shape of a line having a predetermined length； a first fixing member which is disposed at one end of the dental floss； and a second fixing member which is disposed at the other end of the dental floss, and a dental floss holder comprises: a body having a first relative fixing member, to which the first fixing member is fixed, and having a holding part on which a used section of the dental floss is held； and a second relative fixing member disposed at the body, the second fixing member being fixed to the second relative fixing member.
