A surgical laser system can include a fluorescent sensing assembly for detecting fluorescent signal from a tissue under a surgical operation of the surgical laser system. The surgical laser system can include an aiming laser assembly, which can be configured to provide excitation energy for the fluorescent process. The surgical laser system can include an infrared sensing assembly, which can provide temperature related data, for example, to prevent damages to the tissue due to overheating. The surgical laser system can be configured to use the off-time of the surgical laser for tissue sensing. Data from tissue sensing can be further analyzed by an integrated robotic surgical system to provide a highly precise surgical procedure.