John van Wicheren REYNDERS, III,Tirtha CHAKRABORTY,Stephen HOGE,Iain James MCFADYEN
申请号：
US15525826
公开号：
US20170362627A1
申请日:
2015.11.04
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present disclosure provides multiparametric codon optimization methods to improve at least a property in a candidate nucleic acid sequence. Such parameters include improving nucleic acid stability (e.g., mRNA stability), increasing translation efficacy in the target tissue, reducing the number of truncated proteins expressed, improving the folding or prevent misfolding of the expressed proteins, reducing toxicity of the expressed products, reducing cell death caused by the expressed products, and increasing or decreasing protein aggregation. After such optimization, the resulting optimized nucleic acid sequence has at least one optimized property with respect to the candidate nucleic acid sequence.
