A method for producing a composition containing carotenoid at a content of80% or greater, characterized in performing extraction on a culture of amicroorganism,a concentrate of the culture, or a dried substance thereof with at least oneselected fromthe group consisting of lower alcohols, water-containing lower alcohols, lowerdialkylketones and ethers; and then washing a precipitate, obtained byconcentrating theextract solution, with at least one selected from the group consisting oflower alcohols,water-containing lower alcohols, hydrocarbon-based solvents and lowerdialkylketones;and food, a pharmaceutical composition or a cosmetic substance comprising thecarotenoid-containing composition.