Disclosed are an ultrasound diagnostic apparatus and an operating method thereof. The operating method includes setting a region of interest (ROI) intended to be enlarged in an ultrasound image of an object, acquiring ultrasound data corresponding to a candidate region including the ROI, generating a candidate image corresponding to the candidate region, based on the acquired ultrasound data, detecting shaking of a probe, and displaying a portion of a candidate image corresponding to the ROI, based on the detected shaking of the probe.