Sumitomo Chemical Company, Limited;Riken; Limited;Sumitomo Chemical Company
发明人:
Kuwahara, Atsushi,Sasai, Yoshiki
申请号：
AU2014362722
公开号：
AU2014362722A1
申请日:
2014.10.16
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
The present invention provides a method for manufacturing a cell aggregate containing a ciliary marginal zone-like structure, the method comprising a step for culturing a retinal tissue-containing cell aggregate, wherein the percentage of Chx10-positive cells present in the retinal tissue is 20% to 100%, in a serum-free medium or serum medium containing a Wnt signal pathway-affecting substance and a FGF signal pathway-inhibiting substance only in the period until cells expressing RPE65 gene appear, and then the obtained "cell aggregates in which RPE65 gene-expressing cells have not appeared" are cultured in a serum-free medium or serum medium that does not contain a Wnt signal pathway-affecting substance. The manufacturing method according to the present invention makes it possible to manufacture ciliary marginal zone-like structures with high efficiency.
