SUMITOMO CHEMICAL COMPANY, LIMITED;RIKEN; LIMITED;SUMITOMO CHEMICAL COMPANY
发明人:
KUWAHARA, ATSUSHI,SASAI, YOSHIKI
申请号：
SG11201604705U
公开号：
SG11201604705UA
申请日:
2014.10.16
申请国别(地区):
SG
年份:
2016
代理人:
摘要：
The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the "cell aggregate in which a RPE65 gene-expressing cell does not appear" thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.
