THE CHILDREN'S HOSPITAL OF PHILADELPHIA;THE CHILDRENS HOSPITAL OF PHILADELPHIA
发明人:
HIGH, KATHERINE A.,WRIGHT, JOHN FRASER,HAUCK, BERND,QU, GUANG
申请号：
CA2858980
公开号：
CA2858980A1
申请日:
2012.12.12
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
In accordance with the present invention, a method for increasing the yield of rLV vector particles comprising a trans gene encoding a therapeutic protein or fragment thereof is disclosed. In one approach, cells are transfected with plasmids encoding the necessary components for rLV production using a calcium chloride transfection mix at pH 7.1 wherein the calcium chloride and plasmids form a complex which is added to the cells at a constant speed. The cells are then incubated for a suitable time period wherein virus particle media is collected at least twice during the incubation period and stored in a cold storage unit, thereby reducing virus inactivation.
