Described herein is a device (100) for simultaneous spectrally resolved acquisition of all pixels of large-area (up to 1 cm2) samples (103), the device (100) comprising at least:a light source (101),a signal detection unit (102) for measuring the spectroscopic signal,an optical system for directing light in form of a first light path (104) onto the sample (103) and for collecting light that is scattered, emitted or reflected by the sample (103) and guiding the collected light away from the sample towards the signal detection unit (102) in form of a second light path (105), wherein the optical system is characterized in thata fiber bundle (107) is provided for guiding the scattered, emitted or reflected light to the signal detection unit (102), the fibers of the fiber bundle are arranged as fiber matrix (106) at the sample side, and arranged as one row (114) at the side of the signal detection unit (102),a first microlens array (111) is arranged in the first light path (103), whereby the first microlens array (111) focusses the light onto the sample (103) as well as collects the light that is scattered, emitted or reflected by the sample (103), anda second microlens array (110) is arranged in the second light path (105), whereby the second microlens array (110) focusses the collected light individually into single fibers (107, 108) of the fiber matrix (106). Preferably, a square core fiber (126) is provided for guiding the light to the first light path (104). The fiber bundle (107) preferably comprises at least 100 and more preferably at least 400 multimode fibers (108).Described is also the use of the device according to the invention for imaging of large-area (up to 1 cm2) samples using Raman, fluorescence, fluorescence lifetime, and reflectance spectroscopy.The great advantage of the present invention is that simultaneous imaging spectroscopy can be performed on a great number of pixels of the sample under investigation.
