A system for disinfecting a room comprising a spray dispenser which dispenses a solution having antimicrobial properties at predetermined time intervals so as to provide continuous disinfection of the room, in combination with an HVAC system having an air filter which has been treated with a solution also having antimicrobial properties so as to further aid in disinfection of air entering the room. The system is designed to provide around-the-clock disinfection of the room thereby reducing the reliance on personnel to disinfect a room, especially in a healthcare setting such as in an emergency room or a surgical theatre.