CASPER VROEMEN,JACQUELYN A. HUITINK,MARCO VAN BRUSSEL-ZWIJNEN,MARTIJN SILVAN SCHEFFERS,PAULA JOHANNA MARIA TEUNISSEN
申请号：
BR112015002653
公开号：
BR112015002653A2
申请日:
2013.08.13
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
A fungal alpha amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75 C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
