PROBLEM TO BE SOLVED: To provide a method for stably accumulating a target protein in a plant cell or a plant body, and to provide a transgenic plant in which the protein is accumulated.SOLUTION: There is provided the method for accumulating the protein in a plant cell. The method for accumulating the protein in a plant cell includes: expressing a gene encoding the target protein having an intracellular membrane system migration signal at the N-terminal and having a vacuolar migration signal at the C-terminal in a multiple variant in which a myrosin cell lacking an endogenous vacuolar protein is present even in the plant body other than the periphery of a vascular bundle thereby accumulating the target protein or a protein lacking the N-terminal region of the target protein in the interior of the myrosin cell in the multiple variant.COPYRIGHT: (C)2013,JPO&INPIT【課題】目的のタンパク質を、植物細胞や植物体に安定して蓄積させる方法、及び、タンパク質を蓄積させた形質転換植物の提供。【解決手段】タンパク質を植物細胞内に蓄積させる方法であって、液胞内在性タンパク質が欠損したミロシン細胞が、維管束周辺以外の植物体内にも存在している多重変異体に、N末端に細胞内膜系移行シグナルを有し、かつC末端に液胞移行シグナルを有する標的タンパク質をコードする遺伝子を発現させることにより、当該多重変異体内のミロシン細胞の内部に、前記標的タンパク質又は前記標的タンパク質のN末端領域が欠損したタンパク質を蓄積させることを特徴とする、タンパク質の植物細胞内への蓄積方法。【選択図】なし
