A tissue harvesting apparatus is provided which can reliably prevent a worker from being stabbed with a needle tip of a needle tube at the time of disposal. When disposing a used tissue harvesting apparatus used for harvesting of a living body tissue, a sheath member with a puncture pipe body (a puncture needle) inserted and arranged in the inside thereof is wound in the form of a loop around an operating rod and locked in a locking groove. Then, a needle tip of the puncture pipe body protruding from a leading end of the sheath member is housed and fixedly supported in a needle housing hole formed in an end face of a second flange portion on the base end side of the operating rod.