The present disclosure relates to liposomal formulations of aminoglycoside such as gentamicin, the method of making and method of using such formulations. The aminoglycoside liposomal formulations disclosed have higher drug to lipid loading ratio, and enable the formulations to be used to treat infections caused by bacterial species resistant to common antibiotics. In one embodiment, a gentamicin liposome formulation with a gentamicin to phospholipid ratio of 10:1 to 26:1 has been prepared and shown to be effective at treating infections caused by R. equi.
