CHO, HAN JIK,CHOI, YONG UI,HUH, JONG UK,JU, SEON A,KIM, I YEOP,KIM, YEONG SIN,LEE, GANG SEOP,SHIN, JEONG SUN,SIM, OK GYEONG
申请号：
KR20020063095
公开号：
KR20040033824(A)
申请日:
2002.10.16
申请国别(地区):
韩国
年份:
2004
代理人:
摘要：
PURPOSE: A method for mass-propagation of black berry(Rubus fruticosus L.) by using biotechnology technique is provided, therefore,black berry(Rubus fruticosus L.) is mass-propagated at any seasons by using tissue culture technique, and the income of farmhouses is consequently increased. CONSTITUTION: A method for mass-propagation of black berry(Rubus fruticosus L.) by using biotechnology technique comprises the steps of: treating sprouts of black berry(Rubus fruticosus L.) with ethanol for 1 minute and 1.2% sodium hypochlorite for 10 to 15 minutes; culturing the black berry(Rubus fruticosus L.) sprouts on a MS agar medium containing NAA and BAP at 1500 lux and 25 deg. C for 16 hours to induce shoots; and transferring the shoots on MS media having different glycine concentrations from 0 to 30.0 micromole to generate roots and followed by transplantation to the soil and cultivation for 3 weeks.
