The present invention provides tissue engineering equipment for a bio-mimetic tissue structure, which comprises a dielectrophoretic force control device, a light source control device and a 3D-structured colloid preparation device. The implementation method employs the dielectrophoretic force to control the specimen within a controlled area, and conducts continuous cultivation with a continuous perfusion system, followed by employing fluid to slowly perfuse a single-layered algae colloid into the slots on an acrylic sheet for stacking, forming the algae gel as small pieces in 3D structure and taking out and overturning the pieces to become a small piece of bio-mimetic tissue 3D structure.一種仿生物組織結構之組織工程設備,包含有一光介電泳力操控裝置、一光源控制裝置及一立體結構膠體製備裝置,其實施方法係藉由光介電泳力對操控區域內的單元體進行操控,並透過一連續式灌流系統進行連續式的培養,再利用流體將單層海藻膠體緩慢沖入該壓克力板上之凹槽堆疊,使其以海藻凝膠固定成小塊立體結構後取出,再將其翻轉即成為一小塊仿生物組織立體結構。(10)...光介電泳力操控裝置(11)...上層導電玻璃(111)...培養液進液孔(112)...培養液出液孔(12)...下層導電玻璃(123)...單元體(124)...鉬塗層(125)...非晶相矽塗層(13)...微通流道