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METHOD FOR LONG-TERM STORAGE OF POROUS BODY BEARING CHONDROCYTES
专利权人:
FUJISOFT INCORPORATED
发明人:
HARAI, MOTOHIRO,TATEYAMA, TOSHIAKI
申请号:
EP12853525
公开号:
EP2787074A4
申请日:
2012.11.30
申请国别(地区):
EP
年份:
2015
代理人:
摘要:
Disclosed is a method for storing a cartilage-cell-adhering porous body in an airtight state over a long term of at shortest 14 days. The method comprises suspending isolated cartilage cells into atelocollagen at an administration cell concentration of 1.0 × 107 to 1.0 × 108 cells/ml to prepare a cell suspension, seeding the cell suspension to a biocompatible porous body, gelatinizing the cell suspension on the porous body to yield the cartilage-cell-adhering porous body, and keeping the cartilage-cell-adhering porous body at a temperature of 26 to 37°C in a medium having an amount of 1 mL or more per 1.0 × 105 cells that are administrated living cells out of the cartilage cells, containing insulin, a fibroblast growth factor, and a 5% or more serum, while the porous body is shaken to set the shaking speed of the medium in the range of 0 to 2.8 cm/s.
来源网站:
中国工程科技知识中心
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