EVERETT, GABRIEL F.K.,GREENWALD, CHARLES,PRUITT, JUDY,ROSMARIN, AMANDA,CHURCH, JORDAN,ABERLE, DANIEL,ABOAGYE, GEORGE
申请号：
CA3019732
公开号：
CA3019732A1
申请日:
2017.04.05
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/ or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41°C to 44°C for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.
