How to differentiate induce neural crest cell populations from pluripotent stem cells, and the present invention, acquired of cells derived from the neural crest or neural crest disease caused by congenital abnormalities of neural crest cell populations that were induced to differentiate by the method on clinical application in the treatment of the diseases caused by such disorders. It is also related to basic research drug discovery research using cells derived from the neural crest further, such as generation and using the neural crest cells.本発明は、多能性幹細胞から神経堤細胞群を分化誘導する方法、及び前記方法によって分化誘導された神経堤細胞群の先天的な異常による神経堤症もしくは神経堤に由来する細胞の後天的な障害によって引き起こされた疾患への治療等への臨床応用に関する。さらに神経堤に由来する細胞を用いた創薬研究、及び神経堤細胞を用いた発生などの基礎研究にも関連する。
