PROBLEM TO BE SOLVED: To provide a puncture device and a puncture method which reduce a burden on a patient, exhibits high safety of the patient, and are capable of easily embedding an indwelling object for supporting a biological tissue into a living body.SOLUTION: A puncture device 1 comprises: two puncture needles 71 and 81 provided turnably a divided piece 31 put on the puncture needle 71 and a divided piece 32 put on the puncture needle 81. Then, the puncture needles 71 and 81 are turned together to the distal end side to cause the distal end part of the puncture needle 71 and the distal end part of the puncture needle 81 to engage with each other, and thus, the divided pieces 31, 32 can be connected to each other within a living body.COPYRIGHT: (C)2015,JPO&INPIT【課題】患者の負担が少なく、患者の安全性が高く、また、生体組織支持用留置物を容易に生体内に埋設することができる穿刺装置および穿刺方法を提供すること。【解決手段】穿刺装置1は、回動可能に設けられた2本の穿刺針71、81と、穿刺針71に被せられた分割片31と、穿刺針81に被せられた分割片32とを有している。そして、穿刺針71、81を共に先端側へ回動させて穿刺針71の先端部と穿刺針81の先端部とを係合させることで、生体内にて分割片31、32が接続可能となる。【選択図】図2