Disclosed are a test strip and a method for measuring biomedical data. The test strip comprises an upper cover having one or more application holes a lower support having one or more detected parts at positions corresponding to detecting units of the measuring apparatus an erythrocyte and LDL cholesterol-filtering layer designed to filter off both of erythrocytes and LDL cholesterol from an applied blood sample by agglutinating erythrocytes and precipitating LDL cholesterol and a reaction layer in which the blood sample free of erythrocytes and LDL cholesterol is reacted with an reagent, the filtering layer and the reaction layer being stacked between the lower support and the upper cover. Structured to filter off erythrocytes and low-density lipoprotein (LDL) cholesterol from a blood sample in a single layer, the test strip of the present invention can be made to be slim and allows a low volume of blood to be analyzed.