Analyte sensor assemblies comprising: an electrochemical sensor for determining a concentration of an analyte and an electrochemical sensor control unit. The electrochemical sensor comprising a working electrode having a sensing layer and a non-crosslinked cubic phase lyotropic liquid crystal membrane disposed over the sensing layer, the sensing layer comprising an analyte-responsive enzyme, and a counter electrode in communication with the working electrode. And the electrochemical sensor control unit comprising a housing adapted for placement on or implantation in a patients skin, and sensor control unit electronics. Wherein a measured current from an applied potential between the working electrode and the counter electrode correlates to a concentration of an analyte.
