A novel soybean variety, designated 5PWAV53 is provided. Also providedare the seeds of soybean variety 5PWAV53, cells from soybean variety5PWAV53, plants of soybean 5PWAV53, and plant parts of soybean variety5PWAV53. Methods provided include producing a soybean plant by crossingsoybean variety 5PWAV53 with another soybean plant, methods for introgressinga transgenic trait, a mutant trait, and/or a native trait into soybean variety5PWAV53, methods for producing other soybean varieties or plant parts derivedfrom soybean variety 5PWAV53, and methods of characterizing soybean variety5PWAV53. Soybean seed, cells, plants, germplasm, breeding lines, varieties,and plant parts produced by these methods and/or derived from soybean variety5PWAV53 are further provided.
