BORNSCHEUER, UWE T.,WEINER, DAVID PAUL,HITCHMAN, TIM,LYON, JONATHAN,WONGSAKUL, SIRIRUNG
申请号：
CA2515583
公开号：
CA2515583C
申请日:
2004.03.08
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
The invention provides hydrolases, polynucleotides encoding them, and methodsof making and using these polynucleotides and polypeptides. In one aspect, theinvention is directed to polypeptides, e.g., enzymes, having a hydrolaseactivity, e.g., an esterase, acylase, lipase, phospholipase (e.g.,phosphlipase A, B, C and D acitvity, patatin activity, lipid acyl hydrolase(LAH) activity) or protease activity, including thermostable andthermotolerant hydrolase activity, and polynucleotides encoding these enzyme,and making and using these polynucleotides and polypeptides. The hydrolaseactivities of the polypeptides and peptides of the invention include esteraseactivity, lipase activity (hydrolysis of lipids), acidolysis reactions (toreplace an esterified fatty acid with a free fatty acid), transesterificationreactions (exchange of fatty acids between triglycerides), ester synthesis,ester interchange reactions, phospholipase activity and protease activity(hydrolysis of peptide bonds). The polypeptides of the invention can be usedin a variety of pharmaceutical, agricultural and industrial contexts,including the manufacture of cosmetics and nutraceuticals. In another aspect,the polypeptides of the invention are used to synthesize enantiomerically purechiral products.
