Van Den Brink, Johannes Maarten,Lund, Martin,Jacobsen, Jonas,Hansen, Sari Charlotte,Jeppesen, Iben
申请号：
NZ72539813
公开号：
NZ725398A
申请日:
2013.05.22
申请国别(地区):
NZ
年份:
2018
代理人:
摘要：
ABSTRACT The present invention relates to variants of chymosin with improved milk-clotting properties, and methods for making them. In particular, the present invention relates to a method for making an isolated chymosin polypeptide variant comprising the steps: (a): making an alteration in a parent polypeptide having chymosin activity, wherein the alteration comprises the substitution Q300R； and (b): producing and isolating the altered polypeptide of step (a) and thereby obtaining the isolated chymosin polypeptide variant, wherein the variant has chymosin activity； and wherein : (i): the amino acid position of the parent polypeptide is determined by an alignment of the parent polypeptide with the polypeptide of SEQ ID NO: 1 (bovine chymosin) - i.e. the polypeptide of SEQ ID NO : 1 is used to determine the corresponding amino acid sequence in the parent polypeptide； and (ii): the parent polypeptide has at least 70% sequence identity with the mature polypeptide of SEQ ID NO: 1 (bovine chymosin), which is from amino acid position 59 to amino acid position 381 of SEQ ID NO: 1； and wherein said method is not performed within a human body. The present invention also relates to chymosin variants produced by such methods, and to methodsfor making foods or feed products comprising adding an effective amount of the isolated chymosin polypeptide variants.
