GAKEN JOHANNES ADRIANUS,FARZANEH FARZIN,RUSELL STEPHEN JAMES
申请号：
INPCT/2000/627/KOL
公开号：
IN2000KN00627A
申请日:
2000.12.12
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
The present invention concerns methods relating to the expression of genes two or more polypeptides in a cell. In particular, it relates to the expression of multiple gene products as a single cistron. The invention is achieved by constructing a nucleic acid construct comprising a first sequence encoding a first polypeptide (such as a protein subunit) and a second sequence encoding a second polypeptide (a further protein subunit), said first and second sequences being separated by a linker sequence encoding an endoproteas4e cleavage site. Once expressed as a single fusion protein it can be cleaved and processed into its constituent, biologically active, first and second polypeptides. The invention has particular implications with regard to expression of proteins that have multiple subunits (e.g. IL-12) that require expression of the independent subunits at equimolar amounts.
