A novel soybean variety, designated 5PMFH81 is provided. Also providedare the seeds of soybean variety 5PMFH81, cells from soybean variety5PMFH81, plants of soybean 5PMFH81, and plant parts of soybean variety5PMFH81. Methods provided include producing a soybean plant by crossingsoybean variety 5PMFH81 with another soybean plant, methods for introgressinga transgenic trait, a mutant trait, and/or a native trait into soybean variety5PMFH81, methods for producing other soybean varieties or plant parts derivedfrom soybean variety 5PMFH81, and methods of characterizing soybean variety5PMFH81. Soybean seed, cells, plants, germplasm, breeding lines, varieties,and plant parts produced by these methods and/or derived from soybean variety5PMFH81 are further provided.
