The present disclosure relates to a conjugated quantum dot nanoparticles, to methods of making such conjugated quantum dot nanoparticles, and to methods of detecting DNA methylation using such conjugated quantum dot nanoparticles.本發明係關於一種共軛之量子點奈米粒子、製造此類共軛之量子點奈米粒子之方法及使用此類共軛之量子點奈米粒子偵測DNA甲基化之方法。