Provided herein are isolation processes and the associated hardware to allow fluid streams to be isolated from a sterilized system (e.g., a sterile process vessel) that contains a sterile process. The isolation processes described herein allow for continuous removal of fluid streams (e.g., waste streams, liquid containing recombinant therapeutic proteins) from a sterilized system (e.g., a biological manufacturing system), which provides for less manual manipulation of the sterilized system and a decreased risk of contaminating the sterilized system.本發明提供隔離方法和相關硬體以允許從含有無菌過程的滅菌系統(sterilized system)(例如，無菌製程容器)隔離流體流。本文描述的隔離方法允許從滅菌系統(例如，生物製造系統)連續移出流體流(例如，廢料流，含有重組治療性蛋白的液體)，其提供對該滅菌系統較少的手動操作和降低污染該滅菌系統的風險。
