A pet teeth cleaning assembly includes a glove configured to be positioned on a person's hand. The glove includes five finger sleeves, a hand portion and a wrist sleeve. Each of the finger sleeves has perimeter wall extending upwardly from the hand portion and a distal end with respect to the hand portion. A plurality of bristles is provided. Each of a plurality of the finger sleeves has the bristles integrally coupled thereto. Tooth cleaning material is positionable on the bristles and rubbed against teeth of a dog to clean the teeth.