Obshchestvo s ogranichennoj otvetstvennostyu "KSENOPLANT"
发明人:
Zajtsev Vladimir Valentinovich,Зайцев Владимир Валентинович,Bakuleva Nataliya Petrovna,Бакулева Наталия Петровна,Chashchin Ivan Sergeevich,Чащин Иван Сергеевич,Vasilev Maksim Gennadevich,Васильев Макс
申请号：
RU2016112138
公开号：
RU0002619870C1
申请日:
2016.03.31
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention is a method for production of biological implants, characterized in that surgically purified and mechanically fragmented initial bone tissue biomaterial is subjected to two to three freeze-thawing cycles, cleaned in the ultrasonic bath by a solution of 0.1 M ethylenediaminetetraacetic acid and 0.01 M sodium hydroxide, then with a solution of 1M hydrochloric acid and 1M sodium chloride, then treated with a solution of 1M sodium chloride and 0.1 M phosphate buffer with washing by a solution of 0.1 M phosphate buffer to pH 7-8, then with a solution of 0.1% Triton x-100 and 1% sodium dodecyl sulfate, followed by a solution of 0.1-1% trypsin and 0.125-0.3% papain at a ration of 1:1 ratio, after that the biomaterial is processed in an ultrasonic bath in 3% hydrogen peroxide, and then treated with a mixture of ethanol or isopropanol and diethyl ether or chloroform at a ratio of 1:2, then treatment is carried out in supercritical carbon dioxide in an autoclave at a pressure of 75-700 atm and a temperature of 32-50°C at periodic pressure drop below the critical point, at the second stage, distilled water and carbon dioxide are introduced into the autoclave at a ratio of 1:(1-3) at a pressure of 150-350 atm and a temperature of 15-25°C, and after autoclave decompression, the biomaterial is subjected to lyophilization and sterilisation.EFFECT: obtaining of biomaterials for bone tissue regeneration with a high degree of biocompatibility.6 cl, 3 exИзобретение относится к области медицины и представляет собой способ получения биологических имплантатов, характеризующийся тем, что хирургически очищенный и механически фрагментированный исходный биоматериал из костной ткани подвергают двум-трем циклам замораживания-размораживания, проводят очистку в ультразвуковой ванне раствором 0,1 М этилендиаминтетрауксусной кислоты и 0,01 М гидроксида натрия, затем раствором 1М соляной кислоты и 1М хлорида натрия, затем обрабатывают раствором 1М хлорида натрия
