University of Maryland, College Park;Children's National Medical Center;The United States of America, As Represented By The Secretary, Dept. Of Health And Human Services
发明人:
Ayyub Omar Bilal,Behrens Adam Michael,Kofinas Peter,Summar Marshall Lynn,Cabrera-Luque Juan Manuel,Cunningham Gary,Simeonov Anton,Marugan Juan
申请号：
US201414915602
公开号：
US9952199(B2)
申请日:
2014.09.02
申请国别(地区):
美国
年份:
2018
代理人:
McCarter & English, LLP
摘要：
The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.
