A dog waste collection assembly includes a handle that has a downward angle between a grip and a head. Thus, the head can be positioned beneath a dog when the dog is defecating and the grip being gripped. A pair of jaws is each pivotally coupled to the head. Each of the jaws is positionable between a closed and an open position. A pair of bag openers is each coupled to a respective one of the jaws and a bag can be positioned around each of the bag openers. An opening unit is movably coupled to the handle and the opening unit is in mechanical communication with the jaws. The opening unit urges the jaws into the open position when the opening unit is manipulated. Thus, the jaws open the bag for receiving the dog waste.