PROBLEM TO BE SOLVED: To provide means to produce an artificial tissue having a three dimensional structure that may maintain a given fine structure for a given period of time and contains a fine structure.SOLUTION: The present invention relates to a production method of an artificial tissue having a three dimensional structure containing a fine structure comprising: a parenchymal cell tissue preparation step of preparing a plurality of extended parenchymal cell tissues having a cell structure containing parenchymal cells; a nonparenchymal cell tissue preparation step of preparing one or more extended nonparenchymal cell tissues having a cell structure that has the fine structure and contains nonparenchymal cells; a cell tissue lamination step of laminating the plurality of extended parenchymal cell tissues and one or more extended nonparenchymal cell tissues having the fine structure so that each of the nonparenchymal cell tissue is pinched between the extended parenchymal cell tissues; and a contraction step of contracting a laminated body of a cellular structure having a three dimensional structure containing the fine structure obtained in the cellular structure lamination step.SELECTED DRAWING: None【課題】所定の微細構造を一定期間維持し得る、微細構造を含む三次元構造を有する人工組織を作製する手段を提供する。【解決手段】本発明は、微細構造を含む三次元構造を有する人工組織の作製方法であって、実質細胞を含む細胞構造体を有する複数個の伸展した実質細胞組織を準備する、実質細胞組織準備工程;前記微細構造を有する、非実質細胞を含む細胞構造体を有する1又は複数個の伸展した非実質細胞組織を準備する、非実質細胞組織準備工程;前記複数個の伸展した実質細胞組織と前記微細構造を有する1又は複数個の伸展した非実質細胞組織とを、非実質細胞組織のそれぞれが伸展した実質細胞組織の間に挟持されるように積層する、細胞組織積層工程;前記細胞組織積層工程で得られた微細構造を含む三次元構造を有する細胞組織の積層体を収縮させる、収縮工程を含む、前記方法に関する。【選択図】なし