The present invention provides a four-line nylon cutter assembly for a mower, characterized in that a winding surface of a spool (30) is divided upward/downward into a first winding surface (330) and a second winding surface (350) by a separating plate (34) provided in the center part; the first winding surface (330) has first through-holes (33) formed therein so as to face each other; the second winding surface (350) has second through-holes (35) formed therein so as to face each other and to be perpendicular to the first through-holes (33) at an interval of Δh vertically; first and second bushings (62, 66) are installed on coupling parts of an upper case (10) and a lower case (50), respectively; a pair of left and right first bushings (62) are installed to face the first through-holes (33) of the spool (30), respectively; a pair of left and right second bushings (66) are installed to face the second through-holes (35) of the spool (30), respectively, and to be perpendicular to the first bushings (62); the center part of a first nylon cutter (N1) having a predetermined length is positioned in the first through-holes (33), and the same is wound around the first winding surface (330) a plurality of times; both end parts of the first nylon cutter (N1) are exposed to the outside by predetermined lengths through first withdrawing grooves (63) of the first bushings (62); the center part of a second nylon cutter (N2) having a predetermined length is positioned in the second through-holes (35), and the same is wound around the second winding surface (350) a plurality of times; and both end parts of the second nylon cutter (N2) are exposed to the outside by predetermined lengths through second withdrawing grooves (67) of the second bushings (66).