The present invention discloses a freeze-drying mixture powder of platelet rich plasma(PRP) and human mesenchymal stem cell(MSC) growth factors and the method for producing thereof. A method for producing the freeze-drying mixture powder of platelet rich plasma and human mesenchymal stem cell growth factors comprises the following steps of: preparing PRP； cultivating MSC growth factors； freeze-drying and mixing PRP and MSC growth factors. Wherein a weight ratio of the PRP in the freeze-drying mixture powder is over than a weight ratio of the MSC growth factors in the freeze-drying mixture powder. The invention enhances the absorption and safety of human body by using autologous blood and human cells as base material, reduces protein variation by using human platelet serum instead of animal fetal bovine serum, overcomes the metamorphism of the thermal sensitivity and aging by using freeze-drying technology, and customizes the freeze-drying mixture powder to apply in different applications.本發明揭露了一種富含血小板血漿結合人體間質幹細胞分泌因子之混合凍晶粉及其製作方法，其步驟包含：備製富含血小板血漿；培養間質幹細胞分泌因子；凍乾與混合富含血小板血漿與間質幹細胞分泌因子。其中，混合凍晶粉所含之自體血小板血漿之重量百分比大於間質幹細胞分泌因子之重量百分比。本發明使用自體血液及人體細胞作為素材以增進人體吸收效益及安全性，所使用之培養液為人類血小板血清而非動物胎牛血清以減少蛋白變異，並利用凍乾技術克服熱敏性及時效造成的變質問題，同時可客製化混合凍晶粉配比以供後續不同環境使用。
