Provided are a method for preparing a new type of recombinant anti-TNF-α chimeric monoclonal antibody and a use. The method comprises: designing and synthesizing the light chain and heavy chain of a CMAB008 antibody according to the preferred codon of a hamster； constructing a eukaryotic expression vector； transfecting an CHO-CR-GS -/- host cell with a GS knockout； and cultivating the cell using a serum-free culture technique； isolating and purifying； and thereby obtaining a low immunogenic CMAB008 antibody.
