Provided herein are cells, compositions and a method to produce in vitro programmed immune stimulating cells comprising: a) obtaining adipose cells from a subject； b) expanding said cells from step a) in culture so as to yield one or more populations of stem cells； c) differentiating said stem cells from step b) into hematopoetic stem cells, mesenchymal stem cells, connective cells, endothelial cells, cardio cells, osteo cells, muscle, cells and/or soft muscle tissue cells； and d) exposing said differentiated cells from step c) to 3D/4D printed molds of preselected bacterial, virus or particles thereof or exposing said differentiated cells from step c) to bacterial, virus or particles thereof so as to generate immune response triggering surface antigen expression in said differentiated cells from step c), thereby yielding in vitro programmed immune stimulating cells.
