A system for the disinfection of rooms and surfaces including at least one plate which can be associated with a room to be treated and equipped with a memory containing a plurality of identification data of the room and at least one diffuser device including a tank containing a liquid solution and nebulising means associated with the tank and designed to draw out the liquid solution from the tank spraying it in the space. The diffuser device includes a control unit associated with the nebulising means and which can be associated with the plate, designed for receiving from the plate a signal representing identification data of the room and designed for controlling the nebulising means as a function of the signal in such a way as to sanitise the room in an automatic manner.