The present invention relates to a method for manufacturing an artificial tumour-specific T cell receptor, comprising the steps of: providing a tumour specific T cell preparation by the method of the invention isolating an individual tumour-specific T cell from said tumour-specific T cell preparation determining the CDR3 regions of both subunits of said T cell receptor of said isolated individual tumour-specific T cell and preparing an artificial T cell receptor comprising said determined CDR3 regions of both subunits. The present invention further relates to a method for isolating a tumour-specific antigen and the use of the tumour specific T cell preparation of the invention for treating cancer in a patient.
